GlycoFlux™ Human HK1 Overexpression CHO-K1 Cell Line (CAT#: GLJF-0925-JF294)

Description	GlycoFlux™ human HK1 overexpression CHO-K1 cell line is a stable glycoengineered line that constitutively overexpresses hexokinase-1, converting glucose to glucose-6-phosphate at the mitochondrial surface. Increased G6P entry enhances pentose phosphate and hexosamine pathways, enabling controlled boosts in UDP-GlcNAc/CMP-Neu5Ac supply, with applications in optimizing complex N-glycan branching, sialylation, and redox-sensitive secretion.
Product Type	Overexpression Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & DMEM
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	HK1
Full Name	Hexokinase 1
Alternative Name	HK; HKD; HKI; HXK1; NMSR; RP79; HMSNR; CNSHA5; HK1-ta; HK1-tb; HK1-tc; NEDVIBA; hexokinase
Location	10q22.1
Gene ID	3098
Summary	Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.