GlycoFlux™ Human HK2 Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF643)

Description	GlycoFlux™ human HK2 overexpression HepG2 cell line is a stable cell line designed to express hexokinase 2 (HK2), the main skeletal muscle isoform that phosphorylates glucose to glucose-6-phosphate. HK2 localizes to the outer mitochondrial membrane, links glycolysis with oxidative metabolism, and is strongly induced by insulin. Its overexpression is a hallmark of highly proliferative cancer cells with elevated glycolytic flux. This system is valuable for cancer metabolism, insulin signaling, and therapeutic screening of glycolysis inhibitors.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	HK2
Full Name	Hexokinase 2
Alternative Name	HKII; HXK2
Location	2p12
Gene ID	3099
Summary	Hexokinase 2 is the main skeletal muscle isoform responsible for phosphorylating glucose to form glucose-6-phosphate, the initial step in most glucose metabolic pathways. It associates with the outer mitochondrial membrane and its expression responds to insulin levels. Studies in rats indicate its involvement in elevated glycolytic rates in rapidly proliferating cancer cells.