GlycoFlux™ Human HK2 Overexpression HepG2 Cell Line

GlycoFlux™ human HK2 overexpression HepG2 cell line is a stable cell line designed to express hexokinase 2 (HK2), the main skeletal muscle isoform that phosphorylates glucose to glucose-6-phosphate. HK2 localizes to the outer mitochondrial membrane, links glycolysis with oxidative metabolism, and is strongly induced by insulin. Its overexpression is a hallmark of highly proliferative cancer cells with elevated glycolytic flux. This system is valuable for cancer metabolism, insulin signaling, and therapeutic screening of glycolysis inhibitors.

Overexpression Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

DMEM & FBS & Glutamine

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

HK2

Hexokinase 2

HKII; HXK2

2p12

Hexokinase 2 is the main skeletal muscle isoform responsible for phosphorylating glucose to form glucose-6-phosphate, the initial step in most glucose metabolic pathways. It associates with the outer mitochondrial membrane and its expression responds to insulin levels. Studies in rats indicate its involvement in elevated glycolytic rates in rapidly proliferating cancer cells.