GlycoFlux™ Human ILKAP Overexpression Jurkat Cell Line (CAT#: GLJF-0925-JF760)

Description	GlycoFlux™ human ILKAP overexpression Jurkat cell line is a stable glycoengineered line that constitutively overexpresses ILKAP, a PP2C serine/threonine phosphatase that binds integrin-linked kinase (ILK) and restrains ILK-dependent GSK3β signaling. By tuning integrin/ILK-WNT cross-talk, this model links phosphoregulation to ER/Golgi trafficking, glycoenzyme activity, and receptor glycoform maturation in T cells. It enables mechanism-based studies of adhesion-signal control of glycosylation and screening of ILKAP/ILK modulators that normalize immune-receptor glycoforms.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Suspension-growing
Passage Ratio	1:3~1:5
Cell Line	Jurkat cell line
Primary Disease	T-cell acute lymphoblastic leukemia (T-ALL)
Lineage	T Lymphocyte
Lineage Subtype	T-cell acute lymphoblastic leukemia (T-ALL)

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ILKAP
Full Name	Ilk Associated Serine/Threonine Phosphatase
Alternative Name	PP2CD; PPM1O; ILKAP2; ILKAP3; PP2C-DELTA
Location	2q37.3
Gene ID	80895
Summary	The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.