GlycoFlux™ Human LCMT1 Overexpression Jurkat Cell Line (CAT#: GLJF-0925-JF761)

Description	GlycoFlux™ human LCMT1 overexpression Jurkat cell line is a stable glycoengineered line that constitutively overexpresses LCMT1, the methyltransferase that carboxyl-methylates the PP2A catalytic subunit. Elevated LCMT1 biases PP2A holoenzyme assembly and substrate scope, allowing causal mapping of how PP2A tuning impacts OST activity, Golgi residence of glycosyltransferases, and terminal capping on T-cell receptors. This model supports PP2A-pathway targeted screens to restore hypoglycosylation or reshape checkpoint-receptor glycoforms.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Suspension-growing
Passage Ratio	1:3~1:5
Cell Line	Jurkat cell line
Primary Disease	T-cell acute lymphoblastic leukemia (T-ALL)
Lineage	T Lymphocyte
Lineage Subtype	T-cell acute lymphoblastic leukemia (T-ALL)

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	LCMT1
Full Name	Leucine Carboxyl Methyltransferase 1
Alternative Name	LCMT; PPMT1; CGI-68
Location	16p12.1
Gene ID	51451
Summary	LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915).