GlycoFlux™ Human LDHA Overexpression Jurkat Cell Line (CAT#: GLJF-0925-JF762)

Description	GlycoFlux™ human LDHA overexpression Jurkat cell line is a stable glycoengineered line that constitutively overexpresses the A subunit of lactate dehydrogenase, driving pyruvate→lactate conversion and NADH→NAD⁺ oxidation during aerobic glycolysis. Enhanced glycolytic flux rewires hexosamine and nucleotide-sugar pools, altering N-glycan branching and sialylation on immune receptors and secreted cytokines. Use it to model Warburg-like metabolism in T cells and to quantify how LDHA inhibition or lactate load rewrites the glycome.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Suspension-growing
Passage Ratio	1:3~1:5
Cell Line	Jurkat cell line
Primary Disease	T-cell acute lymphoblastic leukemia (T-ALL)
Lineage	T Lymphocyte
Lineage Subtype	T-cell acute lymphoblastic leukemia (T-ALL)

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	LDHA
Full Name	Lactate Dehydrogenase A
Alternative Name	LDHM; GSD11; PIG19; HEL-S-133P
Location	11p15.1
Gene ID	3939
Summary	This gene encodes the A subunit of lactate dehydrogenase enzyme which catalyzes the reversible conversion of pyruvate to lactate with the concomitant oxidation of NADH to NAD in anaerobic glycolysis. The protein is found predominantly in skeletal muscle and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. The human genome contains several non-transcribed pseudogenes of this gene.