GlycoFlux™ Human PCK1 Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF654)

Description	GlycoFlux™ human PCK1 overexpression HepG2 cell line is a stable cell line engineered to express cytosolic phosphoenolpyruvate carboxykinase 1 (PCK1), a gluconeogenic enzyme converting oxaloacetate to phosphoenolpyruvate with GTP. This reaction represents a key control point in hepatic glucose production, regulated by hormones such as insulin and glucocorticoids. Mutations cause PCK1 deficiency and fasting hypoglycemia. This model is highly relevant for studies of gluconeogenesis, diabetes, and energy homeostasis.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PCK1
Full Name	Phosphoenolpyruvate Carboxykinase 1
Alternative Name	PCKDC; PEPCK1; PEPCKC; PEPCK-C
Location	20q13.31
Gene ID	5105
Summary	This gene encodes a cytosolic enzyme that, with GTP, converts oxaloacetate to phosphoenolpyruvate, releasing CO₂ and GDP. It is a key control point in gluconeogenesis and is regulated by hormones and dietary factors. Mutations cause cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isoform is encoded by a separate gene.