GlycoFlux™ Human PCK2 Overexpression HEK293 Cell Line (CAT#: GLJF-0925-JF423)

Description	GlycoFlux™ human PCK2 overexpression HEK293 cell line boosts expression of mitochondrial phosphoenolpyruvate carboxykinase (PCK2), which catalyzes the same step of gluconeogenesis in mitochondria. This line provides a tool for dissecting isoform-specific roles in glucose metabolism and metabolic adaptation.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & DMEM
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PCK2
Full Name	Phosphoenolpyruvate Carboxykinase 2, Mitochondrial
Alternative Name	PEPCK; PEPCK2; mtPCK2; PEPCK-M
Location	14q11.2-q12
Gene ID	5106
Summary	This gene encodes a mitochondrial enzyme involved in gluconeogenesis, converting oxaloacetate to phosphoenolpyruvate. The cytosolic form plays a critical role in liver gluconeogenesis. Alternative splicing produces different isoforms.