GlycoFlux™ Human PDK2 Overexpression Glycoengineered Cell (CAT#: GLJF-0925-JF40)

Description	GlycoFlux™ human PDK2 overexpression glycoengineered cell is a stable cell line that elevates pyruvate dehydrogenase kinase 2 to inhibit PDH via E1α phosphorylation, lowering acetyl-CoA supply. It enables Warburg-shift models linking reduced acetyl-CoA to hexosamine pathway flux, O-GlcNAcylation, and complex N-glycan branching, hormone/insulin signaling studies with glycoprotein readouts, and PDK inhibitor screening using glycan-resolved secretion and receptor sialylation as endpoints.
Product Type	Overexpression Cell Lines
Cell Line	As requested by the client.

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PDK2
Full Name	Pyruvate Dehydrogenase Kinase 2
Alternative Name	PDHK2; PDKII
Location	17q21.33
Gene ID	5164
Summary	This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.