GlycoFlux™ Human PGK1 Overexpression HEK293 Cell Line (CAT#: GLJF-0925-JF431)

Description	GlycoFlux™ human PGK1 overexpression HEK293 cell line stably produces PGK1, a glycolytic enzyme converting 1,3-diphosphoglycerate to 3-phosphoglycerate, while also acting as a moonlighting angiogenic factor in tumors. This system supports studies of hemolytic anemia, neurological impairment, and tumor angiogenesis.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & DMEM
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PGK1
Full Name	Phosphoglycerate Kinase 1
Alternative Name	PGKA; MIG10; HEL-S-68p
Location	Xq21.1
Gene ID	5230
Summary	This glycolytic enzyme catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate and also acts in tumor angiogenesis. It functions as a moonlighting protein and is involved in multiple distinct processes. Deficiency is linked to hemolytic anemia and neurological impairment.