GlycoFlux™ Human PHKA2 Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF668)

Description	GlycoFlux™ human PHKA2 overexpression HepG2 cell line is a stable glycoengineered line that constitutively overexpresses the hepatic α subunit of phosphorylase kinase within the αβγδ holoenzyme. This model elevates liver-mode glycogenolytic control of G1P/UDP-sugar availability to map consequent changes in complex-type branching, core fucosylation, and lipoprotein/apolipoprotein glycoform patterns relevant to GSD-IXA.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKA2
Full Name	Phosphorylase Kinase Regulatory Subunit Alpha 2
Alternative Name	PHK; PYK; XLG; PYKL; XLG2; GSD9A
Location	Xp22.13
Gene ID	5256
Summary	Phosphorylase kinase is assembled from 16 total subunits-four each of alpha, beta, gamma, and delta types. In this form, the hepatic alpha isoform is produced by this gene, while the gamma unit hosts the catalytic site. The delta subunit is a calmodulin that manages calcium dependence. Variants in this gene cause glycogen storage disease type 9A. Several splice forms have been detected but remain incompletely characterized.