GlycoFlux™ Human PHKB Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF669)

Description	GlycoFlux™ human PHKB overexpression HepG2 cell line is a stable glycoengineered line that constitutively overexpresses the regulatory β subunit of phosphorylase kinase shared by liver and muscle isoforms. It enables titration of holoenzyme activity and donor-sugar generation from glycogen, supporting studies of how kinase regulation coordinates with ER/Golgi processing and how PHKB variants perturb glycoprotein maturation (GSD-IXB context).
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKB
Full Name	Phosphorylase Kinase Regulatory Subunit Beta
Location	16q12.1
Gene ID	5257
Summary	This multi-subunit kinase contains the same beta subunit in both liver and muscle isoforms, and it is encoded by this gene. The gamma unit provides enzymatic activity, with the delta (calmodulin) regulating calcium sensitivity. Mutations are linked to glycogen storage disease type 9B. Two pseudogenes are located on chromosomes 14 and 20.