GlycoFlux™ Human PHKG1 Overexpression HEK293T Cell Line (CAT#: GLJF-0925-JF554)

Description	GlycoFlux™ human PHKG1 overexpression HEK293T cell line is a stable glycoengineered line that constitutively overexpresses the γ1 catalytic subunit of phosphorylase kinase. Use it to drive catalytic control of glycogen breakdown and quantify downstream changes in branching/sialylation patterns on receptors and secreted enzymes.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PHKG1
Full Name	Phosphorylase Kinase Catalytic Subunit Gamma 1
Alternative Name	PHKG
Location	7p11.2
Gene ID	5260
Summary	This gene produces a serine/threonine kinase with one kinase domain and two calmodulin-binding domains. As the catalytic component of a 16-subunit phosphorylase kinase complex, it plays a critical role in glycogen breakdown. Pseudogenes exist at 7q11.21 and 11p11.12, and multiple transcript variants have been identified.