GlycoFlux™ Human PPP1R10 Overexpression Jurkat Cell Line (CAT#: GLJF-0925-JF791)

Description	GlycoFlux™ human PPP1R10 overexpression Jurkat cell line is a stable glycoengineered line that constitutively overexpresses PPP1R10 (PNUTS), a PP1-binding scaffold involved in cell-cycle/DNA-repair control and nuclear signaling. By retargeting PP1, it reveals phosphatase control of O-GlcNAc/phosphate crosstalk, chromatin-glycome coupling, and checkpoint impacts on secretory glycoprotein quality.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Suspension-growing
Passage Ratio	1:3~1:5
Cell Line	Jurkat cell line
Primary Disease	T-cell acute lymphoblastic leukemia (T-ALL)
Lineage	T Lymphocyte
Lineage Subtype	T-cell acute lymphoblastic leukemia (T-ALL)

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R10
Full Name	Protein Phosphatase 1 Regulatory Subunit 10
Alternative Name	p99; FB19; R111; CAT53; PNUTS; PP1R10
Location	6p21.33
Gene ID	5514
Summary	This gene encodes a protein phosphatase 1 binding protein. The encoded protein plays a role in many cellular processes including cell cycle progression, DNA repair and apoptosis by regulating the activity of protein phosphatase 1. This gene lies within the major histocompatibility complex class I region on chromosome 6, and alternatively spliced transcript variants have been observed for this gene.