GlycoFlux™ Human PPP1R12A Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF676)

Description	GlycoFlux™ human PPP1R12A overexpression HepG2 cell line is a stable glycoengineered line that constitutively overexpresses MYPT1, the myosin phosphatase targeting subunit. By reshaping actomyosin contractility and vesicle dynamics, it provides a means to quantify how cytoskeletal tone governs Golgi stacking, cargo transit, and glycoform distribution on membrane receptors.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R12A
Full Name	Protein Phosphatase 1 Regulatory Subunit 12A
Alternative Name	MBS; GUBS; M130; MYPT1
Location	12q21.2-q21.31
Gene ID	4659
Summary	Myosin phosphatase target subunit 1 (MBS) is a component of myosin phosphatase, regulating actin-myosin interactions downstream of the small GTPase Rho. Active RhoA binds MBS, influencing myosin light chain (MLC) phosphorylation via Rho-kinase. This phosphorylation reduces myosin phosphatase activity, promoting smooth muscle contraction and actin-myosin association in nonmuscle cells. Overexpression of RhoA or its active form increases MBS and MLC phosphorylation. Several isoforms result from alternative splicing.