GlycoFlux™ Human PPP1R13B Overexpression HEK293 Cell Line (CAT#: GLJF-0925-JF447)

Description	GlycoFlux™ human PPP1R13B overexpression HEK293 cell line is a stable glycoengineered line that constitutively overexpresses ASPP1-family cofactor PPP1R13B (ASPP), which potentiates p53-family transactivation of pro-apoptotic genes. It is ideal for linking p53-driven stress programs to glycome remodeling, death-receptor glycosylation, and secretion of immune mediators, and for assessing ASPP-modulating compounds via glyco-biomarker endpoints.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & DMEM
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R13B
Full Name	Protein Phosphatase 1 Regulatory Subunit 13B
Alternative Name	p85; ASPP1; p53BP2-like
Location	14q32.33
Gene ID	23368
Summary	The encoded ASPP family protein interacts with p53-family members, enhancing their DNA binding and activation of proapoptotic genes. It contains ankyrin repeats and an SH3 domain, and its expression is controlled by E2F transcription factors.