GlycoFlux™ Human PYGL Overexpression HEK293 Cell Line (CAT#: GLJF-0925-JF479)

Description	GlycoFlux™ human PYGL overexpression HEK293 cell line expresses PYGL, the liver isoform of glycogen phosphorylase that releases glucose-1-phosphate from glycogen. Mutations cause Hers disease (glycogen storage disease VI), making this model suitable for liver glycogen metabolism and metabolic disorder studies.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & DMEM
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PYGL
Full Name	Glycogen Phosphorylase L
Alternative Name	GSD6
Location	14q22.1
Gene ID	5836
Summary	The product of this gene is a homodimeric enzyme that releases glucose-1-phosphate from liver glycogen by cleaving alpha-1,4-glucosidic bonds. Conversion from inactive phosphorylase B to active phosphorylase A occurs via phosphorylation at serine 15. Additional regulation is mediated by allosteric effectors and hormones. Mutations cause glycogen storage disease type VI (Hers disease), marked by moderate hypoglycemia, mild ketosis, growth delay, and hepatomegaly. Multiple isoforms are generated by alternative splicing.