Description

GlycoFlux™ Lenti-ALG10 Viral Particle is a pre-packaged, replication-incompetent lentiviral preparation encoding ALG10, a membrane glucosyltransferase that donates the third glucose from dolichyl-phosphate-glucose (Dol-P-Glc) to Glc₂Man₉GlcNAc₂-PP-dolichol, yielding the triglucosylated N-glycan precursor required for ER calnexin/calreticulin quality control. The vector enables durable ALG10 expression in mammalian cells, streamlining creation of stable systems to interrogate lipid-linked oligosaccharide maturation, glycoprotein folding and assembly, rescue of ALG10 loss-of-function backgrounds, and mechanism-focused screening; delivered ready to transduce and compatible with standard antibiotic selection or fluorescence-based enrichment.

Product Type

Recombinant Lentivirus

Target

ALG10

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Full Name	ALG10 alpha-1,2-glucosyltransferase
Alternative Name	ALG10A; DIE2; FLJ14751
Location	12p11.1
Gene ID	84920
Summary	Membrane-associated enzyme adding the third glucose to Glc₂Man₉GlcNAc₂-PP-Dol from Dol-P-Glc, crucial for N-glycan precursor maturation.

GlycoFlux™ Lenti-Human ALG10 Viral Particle (CAT#: GLJF-1025-JF2)

Specifications

Quality Control

Target Information

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