Description

GlycoFlux™ Lenti-ALG12 Viral Particle provides ready-to-transduce, replication-defective lentiviral particles encoding ALG12, a glycosyltransferase 22 family enzyme that catalyzes the α1,6 addition of the eighth mannose to dolichol-PP-Man₇GlcNAc₂, a requisite step toward completion of the lipid-linked N-glycan precursor. It supports stable, long-term ALG12 expression in mammalian models, facilitating construction of pathway reconstruction and complementation assays for LLO elongation defects, evaluation of ER quality-control dependencies, and mechanism-oriented screens; amenable to antibiotic selection workflows or fluorescence sorting without additional vector engineering.

Product Type

Recombinant Lentivirus

Target

ALG12

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Full Name	ALG12 alpha-1,6-mannosyltransferase
Alternative Name	ECM39; CDG1G
Location	22q13.33
Gene ID	79087
Summary	Member of glycosyltransferase 22 family, adding the eighth mannose to dolichol-PP-Man₇GlcNAc₂ via α1,6 linkage, essential for N-glycan precursor completion.

GlycoFlux™ Lenti-Human ALG12 Viral Particle (CAT#: GLJF-1025-JF3)

Specifications

Quality Control

Target Information

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