GlycoFlux™ Lenti-Human ALG3 Viral Particle

GlycoFlux™ Lenti-ALG3 Viral Particle encodes ALG3, an α1,3-mannosyltransferase that adds a mannose to Man₅GlcNAc₂-PP-dolichol, a pivotal step in N-glycan precursor elongation; defects are linked to CDG-Id with abnormal glycosylation and potential neurological symptoms. This ready-to-transduce, replication-incompetent lentiviral construct supports long-term ALG3 expression in mammalian cells, facilitating stable-line generation for LLO chain-extension studies, genotype-phenotype rescue, and ER glycosylation modulator screening; compatible with antibiotic selection and fluorescence-based enrichment.

Recombinant Lentivirus

ALG3

Human

EF1a

Lentivirus

HEK293T

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipped on dry ice with temperature held at or below -80°C.

Store at -80°C to maintain their titer and stability.

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

qPCR

Negative

Sterility testing confirmed no microbial contamination.

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Alg3 Alpha-1,3- Mannosyltransferase

NOT56L; D16Ertd36e; CDGS4; Not56

3q27.1

Key factor in N-glycosylation, attaching alpha-1,3 mannose to Man5GlcNAc2-PP-Dol; defects are associated with CDG-Id, leading to abnormal glycosylation and potential neurological symptoms.