Description

GlycoFlux™ Lenti-ENO3 Viral Particle introduces ENO3 (β-enolase), the adult skeletal-muscle isoform implicated in development/regeneration; this replication-incompetent construct enables creation of durable, stably transduced mammalian cell populations for muscle differentiation models, disease-variant assays, and glycolytic flux studies with routine selection or FACS workflows.

Product Type

Recombinant Lentivirus

Target

ENO3

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Full Name	Enolase 3
Alternative Name	MSE; GSD13
Location	17p13.2
Gene ID	2027
Summary	Beta-enolase is found in adult skeletal muscle and may contribute to muscle development and regeneration. A developmental switch from alpha-enolase to beta-enolase occurs in rodent muscle. Mutations cause glycogen storage disease. Multiple isoforms arise via alternative splicing.

GlycoFlux™ Lenti-Human ENO3 Viral Particle (CAT#: GLJF-1025-JF158)

Specifications

Quality Control

Target Information

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