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GlycoFlux™ Lenti-Human EOGT Viral Particle (CAT#: GLJF-1025-JF32)

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SPECIFIC INQUIRY
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Description GlycoFlux™ Lenti-EOGT Viral Particle encodes EOGT, which transfers N-acetylglucosamine to serine/threonine residues of extracellular proteins-a critical event in N-glycan-related biology. This pre-packaged lentiviral system establishes durable EOGT expression in mammalian cells, enabling stable platforms to examine extracellular glycosylation-dependent signaling, receptor-ligand engagement, and structure-function relationships, and is compatible with routine antibiotic selection or FACS-based enrichment.
Product Type Recombinant Lentivirus
Target EOGT
Species Human
Promotor EF1a
Packaging System Lentivirus
Packaging Cell HEK293T
Application The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.
Full Name EGF domain specific O-linked N-acetylglucosamine transferase
Alternative Name C3orf64; AER61; FLJ33770
Location 3p14.1
Gene ID 285203
Summary Transfers N-acetylglucosamine to serine or threonine residues of extracellular proteins, a critical event in N-linked glycan assembly.
For Research Use Only.
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