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GlycoFlux™ Lenti-Human OGA Viral Particle (CAT#: GLJF-1025-JF87)

Datasheet

SPECIFIC INQUIRY
Antibiotic Resistance:
Fluorescent Reporters:
Titer:
Size:
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Description GlycoFlux™ Lenti-OGA Viral Particle provides OGA, the glycosidase that removes O-GlcNAc from nuclear and cytoplasmic proteins. It is intended for generating stable mammalian cell lines to probe O-GlcNAc cycling, crosstalk with phosphorylation, and target validation in signaling networks; compatible with antibiotic selection or fluorescence enrichment.
Product Type Recombinant Lentivirus
Target OGA
Species Human
Promotor EF1a
Packaging System Lentivirus
Packaging Cell HEK293T
Application The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.
Full Name O-GlcNAcase
Alternative Name NCOAT; MEA5; MGEA5
Location 10q24.32
Gene ID 10724
Summary Removes O-GlcNAc from nuclear and cytoplasmic proteins, modulating their function.
For Research Use Only.
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