Description

GlycoFlux™ Lenti-PCK1 Viral Particle encodes PCK1 (cytosolic PEPCK), which converts oxaloacetate to phosphoenolpyruvate and is hormonally regulated (insulin, glucocorticoids, etc.); defects cause cytosolic PEPCK deficiency. This vector allows routine generation of stable mammalian lines for gluconeogenic flux control, transcriptional regulation assays, and variant rescue under standard selection/FACS.

Product Type

Recombinant Lentivirus

Target

PCK1

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Full Name	Phosphoenolpyruvate Carboxykinase 1
Alternative Name	PCKDC; PEPCK1; PEPCKC; PEPCK-C
Location	20q13.31
Gene ID	5105
Summary	This gene regulates gluconeogenesis by catalyzing the formation of phosphoenolpyruvate from oxaloacetate. It is regulated by insulin, glucocorticoids, and other factors. Defects lead to cytosolic phosphoenolpyruvate carboxykinase deficiency.

GlycoFlux™ Lenti-Human PCK1 Viral Particle (CAT#: GLJF-1025-JF190)

Specifications

Quality Control

Target Information

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