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GlycoFlux™ Lenti-Human PGM3 Viral Particle (CAT#: GLJF-1025-JF201)

Datasheet

SPECIFIC INQUIRY
Antibiotic Resistance:
Fluorescent Reporters:
Titer:
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Description GlycoFlux™ Lenti-PGM3 Viral Particle delivers PGM3, a phosphohexose-mutase-family enzyme noted for mediating glycogen formation and utilization via glucose-1-phosphate/glucose-6-phosphate interconversion (multiple isoforms reported). The ready-to-transduce construct enables construction of stably transduced mammalian cells to interrogate pathway routing, SNP/isoform effects, and stress responses, using routine selection or FACS sorting.
Product Type Recombinant Lentivirus
Target PGM3
Species Human
Promotor EF1a
Packaging System Lentivirus
Packaging Cell HEK293T
Application The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.
Full Name Phosphoglucomutase 3
Alternative Name AGM1; PAGM; IMD23; PGM 3
Location 6q14.1
Gene ID 5238
Summary This gene encodes a member of the phosphohexose mutase family, mediating both glycogen formation and utilization by interconverting glucose-1-phosphate and glucose-6-phosphate. A SNP in this gene may affect diabetic nephropathy resistance. Multiple isoforms exist due to alternative splicing.
For Research Use Only.
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