Description

GlycoFlux™ Lenti-PHKA1 Viral Particle encodes the phosphorylase kinase α (muscle) subunit, a regulatory component of the αβγδ holoenzyme; mutations cause GSD IXd. The replication-incompetent vector is intended for generating stable mammalian cell lines to study skeletal-muscle glycogenolysis control, Ca²⁺/calmodulin coupling, and variant rescue under standard selection or FACS workflows.

Product Type

Recombinant Lentivirus

Target

PHKA1

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Full Name	Phosphorylase Kinase Regulatory Subunit Alpha 1
Alternative Name	PHKA
Location	Xq13.1
Gene ID	5255
Summary	Phosphorylase kinase is a complex with subunits alpha, beta, gamma, and delta. The alpha subunit has isoforms in muscle and liver, with the skeletal muscle isoform encoded by this gene. Mutations cause glycogen storage disease type 9D. Several isoforms exist due to alternative splicing.

GlycoFlux™ Lenti-Human PHKA1 Viral Particle (CAT#: GLJF-1025-JF203)

Specifications

Quality Control

Target Information

Related products