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GlycoFlux™ Lenti-Human PHKB Viral Particle (CAT#: GLJF-1025-JF205)

Datasheet

SPECIFIC INQUIRY
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Titer:
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Description GlycoFlux™ Lenti-PHKB Viral Particle provides the phosphorylase kinase β subunit, shared between liver and muscle isoforms; mutations cause GSD IXb affecting both tissues. The vector is configured to create long-term, stably transduced mammalian cell populations for holoenzyme assembly studies, phosphorylation signaling, and genotype-phenotype testing using selection/FACS enrichment.
Product Type Recombinant Lentivirus
Target PHKB
Species Human
Promotor EF1a
Packaging System Lentivirus
Packaging Cell HEK293T
Application The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.
Full Name Phosphorylase Kinase Regulatory Subunit Beta
Location 16q12.1
Gene ID 5257
Summary Phosphorylase kinase is a 16-subunit enzyme containing alpha, beta, gamma, and delta subunits. The alpha subunit encodes muscle and liver isoforms via separate genes. The beta subunit, encoded by this gene, is the same in both forms. The gamma subunit contains the catalytic site, and the delta subunit (a calmodulin) controls calcium sensitivity. Mutations cause glycogen storage disease type 9B, affecting liver and muscle. Pseudogenes are found on chromosomes 14 and 20.
For Research Use Only.
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