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GlycoFlux™ Lenti-Human PHKG1 Viral Particle (CAT#: GLJF-1025-JF206)

Datasheet

SPECIFIC INQUIRY
Antibiotic Resistance:
Fluorescent Reporters:
Titer:
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Description GlycoFlux™ Lenti-PHKG1 Viral Particle introduces PHKG1, a Ser/Thr kinase bearing two calmodulin-binding regions and serving as the γ catalytic subunit of phosphorylase kinase essential for glycogen breakdown. This construct is optimized to produce stable mammalian transductants for contractile metabolism models, catalytic-site interrogation, and disease-variant analysis, with routine selection or marker-guided isolation.
Product Type Recombinant Lentivirus
Target PHKG1
Species Human
Promotor EF1a
Packaging System Lentivirus
Packaging Cell HEK293T
Application The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.
Full Name Phosphorylase Kinase Catalytic Subunit Gamma 1
Alternative Name PHKG
Location 7p11.2
Gene ID 5260
Summary This gene encodes a Ser/Thr protein kinase with one kinase domain and two calmodulin-binding domains. It is the catalytic subunit of a 16-subunit phosphorylase kinase complex essential for glycogen breakdown. Pseudogenes exist on chromosomes 7q11.21 and 11p11.12. Multiple transcript variants have been identified.
For Research Use Only.
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