Description

GlycoFlux™ Lenti-PPP1R13B Viral Particle introduces PPP1R13B (ASPP1/2 family), a regulator that binds p53-family members to enhance DNA binding and pro-apoptotic gene activation (ankyrin repeats, SH3 domain; E2F-regulated). This pre-packaged vector enables construction of stably transduced mammalian cells for apoptosis pathway reconstruction, transcriptional assays, and allele-effect studies using selection/FACS.

Product Type

Recombinant Lentivirus

Target

PPP1R13B

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Full Name	Protein Phosphatase 1 Regulatory Subunit 13B
Alternative Name	p85; ASPP1; p53BP2-like
Location	14q32.33
Gene ID	23368
Summary	The encoded ASPP family protein interacts with p53-family members, enhancing their DNA binding and activation of proapoptotic genes. It contains ankyrin repeats and an SH3 domain, and its expression is controlled by E2F transcription factors.

GlycoFlux™ Lenti-Human PPP1R13B Viral Particle (CAT#: GLJF-1025-JF215)

Specifications

Quality Control

Target Information

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