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| Description | GlycoFlux™ Lenti-PPP1R16A Viral Particle delivers PPP1R16A, a MYPT-family subunit that targets PP1c to myosin light chain and-distinct from other MYPTs-activates PP1c upon PKA phosphorylation. The replication-incompetent vector enables stable mammalian line generation to study contractility set-points, cAMP-dependent regulation, and phosphatase targeting, with easy adoption of antibiotic selection or fluorescence-based isolation. |
| Product Type | Recombinant Lentivirus |
| Target | PPP1R16A |
| Species | Human |
| Promotor | EF1a |
| Packaging System | Lentivirus |
| Packaging Cell | HEK293T |
| Application | The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy. |
| Shipping | Shipped on dry ice with temperature held at or below -80°C. |
| Storage | Store at -80°C to maintain their titer and stability. |
| Handling Notes | Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection. |
| Product Disclaimer | These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application. |
| Titer Assay | qPCR |
| Mycoplasma Test | Negative |
| Sterility | Sterility testing confirmed no microbial contamination. |
| Transduction Evaluation | On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function. |
| Insert Identity Confirmation | All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity. |
| Full Name | Protein Phosphatase 1 Regulatory Subunit 16A |
| Alternative Name | MYPT3 |
| Location | 8q24.3 |
| Gene ID | 84988 |
| Summary | This MYPT subunit directs PP1c to myosin light chain targets, activating PP1c upon phosphorylation by protein kinase A, unlike other MYPT members whose phosphorylation inhibits PP1c. |