Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ Lenti-SIGLEC15 Viral Particle delivers SIGLEC15, a membrane receptor linked to actin organization and osteoclast biology (bone resorption). Purpose-designed to establish stable mammalian expression lines, this construct enables osteoclastogenesis models, signaling dissection, and inhibitor testing, with straightforward antibiotic selection or FACS-based isolation.

Product Type

Recombinant Lentivirus

Target

SIGLEC15

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Insert Identity Confirmation

All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Target Information

Full Name

Sialic Acid Binding Ig Like Lectin 15

Alternative Name

CD33L3; HsT1361; SIGLEC-15; Sialic Acid Binding Ig Like Lectin

Location

18q21.1

Gene ID

284266

Summary

This protein is predicted to regulate actin cytoskeleton organization, bone resorption, and osteoclast development. It is expected to be located in the membrane and to be part of a protein-containing complex, functioning actively in the plasma membrane.

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GlycoFlux™ Lenti-Human SIGLEC15 Viral Particle