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GlycoFlux™ MMLV-Human EOGT Viral Particle (CAT#: GLJF-1025-JF289)

Datasheet

SPECIFIC INQUIRY
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Description GlycoFlux™ MMLV-EOGT Viral Particle delivers EOGT, which transfers O-GlcNAc to serine/threonine residues on secreted/extracellular EGF-repeat proteins; the replication-defective vector establishes stable mammalian expression lines for Notch-pathway glycosylation, ligand recognition, and secretion-quality control studies.
Product Type Recombinant MMLV Retrovirus
Target EOGT
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name EGF domain specific O-linked N-acetylglucosamine transferase
Alternative Name C3orf64; AER61; FLJ33770
Location 3p14.1
Gene ID 285203
Summary Transfers N-acetylglucosamine to serine or threonine in proteins destined for secretion, an essential step in N-glycan assembly.
For Research Use Only.
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