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GlycoFlux™ MMLV-Human EPM2A Viral Particle (CAT#: GLJF-1025-JF416)

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SPECIFIC INQUIRY
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Description GlycoFlux™ MMLV-EPM2A Viral Particle delivers EPM2A (laforin), a dual-specificity phosphatase that prevents glycogen hyperphosphorylation and Lafora body formation; the system builds stable mammalian cells for glycogen quality-control and Lafora disease complementation.
Product Type Recombinant MMLV Retrovirus
Target EPM2A
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name Epm2A Glucan Phosphatase, Laforin
Alternative Name EPM2; MELF; MELF2
Location 6q24.3
Gene ID 7957
Summary The product of this gene is a dual-specificity phosphatase that prevents over-phosphorylation of glycogen, thereby avoiding insoluble aggregate formation. Loss-of-function mutations lead to Lafora disease, a fatal epilepsy with Lafora body accumulation.
For Research Use Only.
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