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GlycoFlux™ MMLV-Human GANC Viral Particle (CAT#: GLJF-1025-JF422)

Datasheet

SPECIFIC INQUIRY
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Description GlycoFlux™ MMLV-GANC Viral Particle encodes GANC, a GH31 α-glucosidase acting in glycogen remodeling (15q15 diabetes-linked locus); it enables stable line construction for glycogen turnover kinetics and allele-function evaluation.
Product Type Recombinant MMLV Retrovirus
Target GANC
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name Glucosidase Alpha, Neutral C
Location 15q15.1
Gene ID 2595
Summary This glycosyl hydrolase enzyme cleaves alpha-D-glucose residues from glycogen and plays a role in glycogen metabolism. It maps to a region on chromosome 15q15 associated with diabetes susceptibility. Multiple isoforms exist.
For Research Use Only.
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