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GlycoFlux™ MMLV-Human HK2 Viral Particle (CAT#: GLJF-1025-JF436)

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SPECIFIC INQUIRY
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Description GlycoFlux™ MMLV-HK2 Viral Particle introduces HK2, an insulin-responsive hexokinase enriched in muscle and upregulated in many tumors; the ready-to-transduce system enables stable expression in mammalian cells to study Warburg metabolism, nutrient signaling, and inhibitor sensitivity.
Product Type Recombinant MMLV Retrovirus
Target HK2
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name Hexokinase 2
Alternative Name HKII; HXK2
Location 2p12
Gene ID 3099
Summary This gene encodes hexokinase 2, found mainly in skeletal muscle and insulin-responsive. It plays a role in glycolysis and is implicated in the increased glycolysis observed in cancer cells.
For Research Use Only.
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