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GlycoFlux™ MMLV-Human PCK1 Viral Particle (CAT#: GLJF-1025-JF447)

Datasheet

SPECIFIC INQUIRY
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Description GlycoFlux™ MMLV-PCK1 Viral Particle provides cytosolic PCK1 (PEPCK-C), converting oxaloacetate → phosphoenolpyruvate to drive gluconeogenesis; this system builds stable mammalian cell lines for hepatic glucose-output modeling and hormonal regulation studies.
Product Type Recombinant MMLV Retrovirus
Target PCK1
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name Phosphoenolpyruvate Carboxykinase 1
Alternative Name PCKDC; PEPCK1; PEPCKC; PEPCK-C
Location 20q13.31
Gene ID 5105
Summary This gene encodes an enzyme critical for gluconeogenesis, converting oxaloacetate to phosphoenolpyruvate. It is regulated by insulin, glucocorticoids, and diet. Defects result in phosphoenolpyruvate carboxykinase deficiency.
For Research Use Only.
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