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GlycoFlux™ MMLV-Human PPP1R13L Viral Particle (CAT#: GLJF-1025-JF473)

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SPECIFIC INQUIRY
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Description GlycoFlux™ MMLV-PPP1R13L Viral Particle delivers PPP1R13L (iASPP), a conserved p53 inhibitor counterbalancing ASPP1/2; this vector creates durable, stably transduced mammalian lines to probe checkpoint restraint, apoptosis tuning, and oncogenic cooperation.
Product Type Recombinant MMLV Retrovirus
Target PPP1R13L
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name Protein Phosphatase 1 Regulatory Subunit 13 Like
Alternative Name RAI; RAI4; ARCME; CMAEA; IASPP; NKIP1
Location 19q13.32
Gene ID 10848
Summary IASPP is a conserved negative regulator of p53, in contrast to ASPP1 and ASPP2, which activate p53.
For Research Use Only.
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