GlycoFlux™ MMLV-Human SIGLEC12 Viral Particle

GlycoFlux™ MMLV-SIGLEC12 Viral Particle provides SIGLEC12, a CD33-related lectin with ITIM/SLAM-like motifs that recruits SHP1/2; this tool builds durable mammalian expression lines to quantify inhibitory signaling thresholds and endocytic routing.

Recombinant MMLV Retrovirus

SIGLEC12

Human

MMLV 5' LTR promoter

Retrovirus

HEK293T

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipped on dry ice with temperature held at or below -80°C.

Store at -80°C to maintain their titer and stability.

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

qPCR

Negative

Sterility testing confirmed no microbial contamination.

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Sialic Acid Binding Ig Like Lectin 12

S2V; SLG; SIGLECL1; Siglec-XII; Sialic Acid Binding Ig Like Lectin

19q13.41

This gene encodes a member of the SIGLEC3-like subfamily, characterized by extracellular V-set immunoglobulin-like domains, followed by two C2-set domains, along with cytoplasmic ITIM and SLAM-like motifs. Upon tyrosine phosphorylation, the protein recruits SHP1 and SHP2, functioning as an inhibitory receptor in macrophage signaling regulation. The gene is located on 19q13.4, with multiple transcript variants due to alternative splicing.