Description

GlycoFlux™ MMLV-SIGLEC12 Viral Particle provides SIGLEC12, a CD33-related lectin with ITIM/SLAM-like motifs that recruits SHP1/2; this tool builds durable mammalian expression lines to quantify inhibitory signaling thresholds and endocytic routing.

Product Type

Recombinant MMLV Retrovirus

Target

SIGLEC12

Species

Human

Promotor

MMLV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Full Name	Sialic Acid Binding Ig Like Lectin 12
Alternative Name	S2V; SLG; SIGLECL1; Siglec-XII; Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	89858
Summary	This gene encodes a member of the SIGLEC3-like subfamily, characterized by extracellular V-set immunoglobulin-like domains, followed by two C2-set domains, along with cytoplasmic ITIM and SLAM-like motifs. Upon tyrosine phosphorylation, the protein recruits SHP1 and SHP2, functioning as an inhibitory receptor in macrophage signaling regulation. The gene is located on 19q13.4, with multiple transcript variants due to alternative splicing.

GlycoFlux™ MMLV-Human SIGLEC12 Viral Particle (CAT#: GLJF-1025-JF357)

Specifications

Quality Control

Target Information

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