GlycoFlux™ MMLV-Human SIGLEC5 Viral Particle

GlycoFlux™ MMLV-SIGLEC5 Viral Particle delivers SIGLEC5, a CD33-related inhibitory lectin that dampens monocyte/macrophage/neutrophil activation and contributes to immune evasion; the vector supports stable line generation for ligand mapping and host-pathogen interface studies.

Recombinant MMLV Retrovirus

SIGLEC5

Human

MMLV 5' LTR promoter

Retrovirus

HEK293T

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipped on dry ice with temperature held at or below -80°C.

Store at -80°C to maintain their titer and stability.

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

qPCR

Negative

Sterility testing confirmed no microbial contamination.

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Sialic Acid Binding Ig Like Lectin 5

CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5; Sialic Acid Binding Ig Like Lectin

19q13.41

The gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins feature structural motifs in their immunoglobulin-like domains, along with sialic acid recognition sites in the first Ig V-set domain. The encoded protein belongs to the CD33-related subgroup of Siglecs and inhibits the activation of monocytes, macrophages, and neutrophils. It also contributes to immune evasion by Group B Streptococcus (GBS).