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| Description | GlycoFlux™ MMLV-STT3B Viral Particle delivers STT3B, a core oligosaccharyltransferase catalytic subunit responsible for adding N-glycan chains to asparagine sites (defects cause CDG type IX); the replication-defective MMLV vector is engineered to establish stable mammalian expression lines (dividing cells) for OST site-occupancy control, post-translational glycosylation studies, and variant rescue. |
| Product Type | Recombinant MMLV Retrovirus |
| Target | STT3B |
| Species | Human |
| Promotor | MMLV 5' LTR promoter |
| Packaging System | Retrovirus |
| Packaging Cell | HEK293T |
| Application | This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research. |
| Shipping | Shipped on dry ice with temperature held at or below -80°C. |
| Storage | Store at -80°C to maintain their titer and stability. |
| Handling Notes | Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection. |
| Product Disclaimer | These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application. |
| Titer Assay | qPCR |
| Mycoplasma Test | Negative |
| Sterility | Sterility testing confirmed no microbial contamination. |
| Transduction Evaluation | On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function. |
| Insert Identity Confirmation | Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration. |
| Full Name | STT3 oligosaccharyltransferase complex catalytic subunit B |
| Alternative Name | SIMP; STT3-B; FLJ90106 |
| Location | 3p23 |
| Gene ID | 201595 |
| Summary | Core enzyme of the oligosaccharyltransferase complex responsible for adding sugar chains to asparagine residues; defects cause CDG type IX. |