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GlycoFlux™ MSCV-Human EOGT Viral Particle (CAT#: GLJF-1025-JF546)

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SPECIFIC INQUIRY
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Description GlycoFlux™ MSCV-EOGT Viral Particle employs Murine Stem Cell Virus (MSCV) transduction to drive EOGT, transferring N-acetylglucosamine to serine/threonine on secreted/EGF-repeat proteins; the genome-integrating system secures persistent expression in hematopoietic/lymphoid progenitors for secretory O-GlcNAc pathway studies.
Product Type Recombinant MSCV Retrovirus
Target EOGT
Species Human
Promotor MSCV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.
Shipping Dispatched on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to preserve titer and stability.
Handling Notes Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.
Full Name EGF domain specific O-linked N-acetylglucosamine transferase
Alternative Name C3orf64; AER61; FLJ33770
Location 3p14.1
Gene ID 285203
Summary Mediates N-acetylglucosamine transfer to serine/threonine residues on secreted proteins, an important step in N-glycan formation.
For Research Use Only.
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