GlycoSens™ ATP Assay Kit, Phosphomolybdate Method

The GlycoSens™ ATP Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of ATP levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Respiration and Energy Metabolism Series

ATP

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

50 μL

Quantification of ATP is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Adenosine Triphosphate

ATP; Adenosine Triphosphate

Functioning at the core of various biological processes, ATP is a key subject of biochemical analysis. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. As a result, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Specifically engineered for evaluating ATP dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.