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GlycoSens™ ATP Assay Kit, WST Method

CAT#: GLJF-0926-HX1313

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Specifications
Product Description
The GlycoSens™ ATP Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target ATP in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Other Series
Target
ATP
Kit Components
Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
5 μL - 20 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of atp. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect ATP content in the sample.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Adenosine Triphosphate
Alternate Names
ATP; Adenosine Triphosphate
Application Notes
Background
Functioning at the core of various biological processes, ATP is a key subject of biochemical analysis. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. Monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.
Application
Designed for comprehensive functional analysis of ATP within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
Key Features & Advantages
Linear Dynamic Range; Excellent Reproducibility; Ready-to-Use Reagents
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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