GlycoSens™ Calcium/Magnesium-Dependent Adenosine Triphosphatase (Ca²⁺/Mg²⁺-ATPase) Assay Kit, Micro-method

The GlycoSens™ Calcium/Magnesium-Dependent Adenosine Triphosphatase (Ca²⁺/Mg²⁺-ATPase) Assay Kit delivers a robust micro-volume spectrophotometric protocol for assessing Calcium/Magnesium-Dependent Adenosine Triphosphatase (Ca²⁺/Mg²⁺-ATPase) activity. The optimized reaction system minimizes background interference for streamlined workflow efficiency.

Oxidative Phosphorylation Series

Ca²⁺/Mg²⁺-ATPase

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

50 μL

Ca²⁺ Mg²⁺-ATPase breaks down ATP to produce ADP and inorganic phosphorus. The activity of ATPase is determined by measuring the amount of inorganic phosphorus.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Calcium/Magnesium-Dependent Adenosine Triphosphatase

Ca²⁺/Mg²⁺-ATPase; Calcium/Magnesium-Dependent Adenosine Triphosphatase Standard; Calcium/Magnesium-Dependent Adenosine Triphosphatase Reagent

Calcium/Magnesium-dependent adenosine triphosphatase (Ca²⁺/Mg²⁺-ATPase) acts as an essential biochemical entity within diverse cellular pathways. It plays a fundamental role in regulating intracellular ion concentrations and overall membrane potential. Consequently, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Specifically engineered for evaluating calcium/magnesium-dependent adenosine triphosphatase (Ca²⁺ Mg²⁺-ATPase) dynamics in membrane biology, ion homeostasis, and cell signaling research.

Stable Signal Output; Broad Sample Compatibility; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.