GlycoSens™ Mitochondrial Respiratory Chain Complex V Assay Kit, Spectrophotometry

The GlycoSens™ Mitochondrial Respiratory Chain Complex V Assay Kit offers a highly reliable and sensitive spectrophotometric platform for quantifying mitochondrial respiratory chain complex V across varied biological matrices. Designed for rapid operation and high precision, this assay ensures reproducible quantification with low sample volume requirements.

Oxidative Phosphorylation Series

Mitochondrial Respiratory Chain Complex V

Mitochondrial Isolation Buffer; Lysis Reagent; Protease Inhibitor Cocktail; Wash Buffer

Isolated mitochondria, cell lysates, tissue homogenates

50 μL

Complex V hydrolyzes ATP to yield ADP and inorganic phosphate (Pi). Complex V catalytic activity is calculated by measuring the rate of inorganic phosphate formation colorimetrically.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Respiratory Chain Complex V

Complex V; Mitochondrial Respiratory Chain Complex V Standard; Mitochondrial Respiratory Chain Complex V Reagent; ATP Synthase

Mitochondrial respiratory chain complex V acts as an essential biochemical entity within diverse cellular pathways. It plays a critical role in cellular bioenergetics by maintaining the proton motive force across the inner mitochondrial membrane. Moreover, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Optimized for high-throughput profiling of mitochondrial respiratory chain complex V in tissue bioenergetics profiling, ATP synthasome kinetics, and biochemical pathway modeling.

High Specificity; Broad Sample Compatibility; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.