GlycoSens™ Polyphenol Oxidase (PPO) Assay Kit

The GlycoSens™ Polyphenol Oxidase (PPO) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target polyphenol oxidase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

PPO

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of polyphenol oxidase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect PPO content in the sample.

Within 1 hour

420 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Polyphenol Oxidase

Polyphenol Oxidase; PPO; Polyphenol Oxidase Standard; Polyphenol Oxidase Reagent

Polyphenol oxidase (PPO) acts as an essential biochemical entity within diverse cellular pathways. Through its catalytic activity, it ensures the safe processing of oxidative metabolites within the cell. Moreover, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Widely applicable for tracking polyphenol oxidase (PPO) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

Optimized Reaction Dynamics; Excellent Reproducibility; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.