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GlycoSens™ Sodium/Potassium-Transporting Adenosine Triphosphatase (Na+/K+-ATPase) Assay Kit

CAT#: GLJF-0926-HX134

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Specifications
Product Description
The GlycoSens™ Sodium/Potassium-Transporting Adenosine Triphosphatase (Na+/K+-ATPase) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Product Type
Oxidation and Antioxidant Series
Target
Na+/K+-ATPase
Kit Components
Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution
Sample Types
Tissue homogenates, cell lysates, biological fluids
Sample Volume
50 μL
Detection Principle
Quantification of sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Sodium/Potassium-Transporting Adenosine Triphosphatase
Alternate Names
Sodium/Potassium-Transporting Adenosine Triphosphatase; Na+/K+-ATPase; Sodium/Potassium-Transporting Adenosine Triphosphatase Standard; Sodium/Potassium-Transporting Adenosine Triphosphatase Reagent
Application Notes
Background
The presence and activity of sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) are closely linked to overall organismal health. By driving active ion exchange, it supports electrical excitability and secondary active transport processes. Thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.
Application
Tailored for accurate quantification of sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) across cell biology, organelle bioenergetics, and intracellular signaling cascades.
Key Features & Advantages
Optimized Reaction Dynamics; Excellent Reproducibility; Minimized Interference
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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