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The GlycoSens™ Sodium/Potassium-Transporting Adenosine Triphosphatase (Na+/K+-ATPase) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Quantification of sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
The presence and activity of sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) are closely linked to overall organismal health. By driving active ion exchange, it supports electrical excitability and secondary active transport processes. Thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.
Application
Tailored for accurate quantification of sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) across cell biology, organelle bioenergetics, and intracellular signaling cascades.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.