GlycoSens™ Soil Acid Protease (S-ACPT) Assay Kit, Micro-method

The GlycoSens™ Soil Acid Protease (S-ACPT) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of soil acid protease. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Soil Series

S-ACPT

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Soil, sediments, solid substrates

20 μL - 100 μL

Target soil acid protease (S-ACPT) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target S-ACPT.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Soil Acid Protease

Soil Acid Protease; S-ACPT

Functioning at the core of various biological processes, soil acid protease (S-ACPT) is a key subject of biochemical analysis. It acts as a primary indicator of soil microbial activity and biogeochemical cycling. Assessing this component offers a reliable parameter for agricultural research or biological studies.

Ideal for investigating soil acid protease (S-ACPT) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.