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The GlycoSens™ Sucrose Phosphate Synthase (SPS) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of sucrose phosphate synthase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Target sucrose phosphate synthase (SPS) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target SPS.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
In the context of biological research, sucrose phosphate synthase (SPS) has emerged as a molecule of high interest. Its enzymatic function is indispensable for the efficient generation of cellular biomass and storage reserves. Moreover, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.
Application
Ideal for investigating sucrose phosphate synthase (SPS) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
Key Features & Advantages
Stable Signal Output; Broad Sample Compatibility; Stringent Quality Control
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.