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Creative Biolabs is a leading company that has established a comprehensive and advanced glycosylation analysis platform. Based on the latest technologies, we are capable of offering comprehensive monosaccharides analysis services. Our professional scientists are happy to share our knowledge and passion in this field to facilitate worldwide customers' research and project development.
N-glycans and O-glycans consist of various monosaccharides including fucose (Fuc), mannose (Man), N-acetylglucosamine (GlcNAc), N-acetlygalactosamine (GalNAc), galactose (Gal), glucose (Glc) and sialic acids. The number of monosaccharides can be quantified by monosaccharide composition analysis. Besides, it can also detect changes in glycosylation. Monosaccharide analysis is, therefore, a critical and essential way to determine the composition of complex carbohydrates. Moreover, monosaccharide analysis can also be used as an exploratory technique for identifying various monosaccharide modifications (including sulfation and phosphorylation).
Our platform provides a serious of technologies for monosaccharide analysis including but not limited to high-performance anion-exchange chromatography with pulsed amperometric detection(HPAE-PAD), high-performance liquid chromatography (HPLC), mass spectrometry (MS), and capillary electrophoresis (CE).
HPAE-PAD is a common technology used for glycoprotein glycosylation analysis. The principle of HPAE-PAD is separating carbohydrates with specific interactions between their carboxyl and hydroxyl groups based on composition, size, linkages, charge, and isomerism. HPAE-PAD is a kind of advanced method which can reduce analyst time, expense, and exposure to hazardous chemicals as it doesn't need sample derivatization. Samples are usually releasing the monosaccharides in the acid hydrolyzed way, and then lyophilized, dissolved in water, finally analyzed by HPAE-PAD. Creative Biolabs has accumulated extensive experience in applying HPAE-PAD for monosaccharide analysis. We are happy to offer high-quality custom services by adjusting protocols to meet even the most specific requirements in monosaccharide analysis.
Fig.1 Monosaccharide analysis by HPAE-PAD. (Zhang, 2012)
HPLC combined with the fluorescent labeling method can stably and adequately determine monosaccharides. Generally, glycoprotein will release monosaccharides in a mild acid hydrolysis procedure. Next, 2-aminobenzoic acid (2AA) is usually used to label the released monosaccharides. At this time, these released monosaccharides are fluorescent, which can be relative quantitatively analyzed by HPLC. Creative Biolabs is proficient at employing HPLC for carbohydrate analysis, and thus you can trustingly hand over any problem to us.
MS, in particular liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), has evolved as one of the most powerful tools for glycan analysis. Creative Biolabs has long been committed to MS technology and confident to provide the most comprehensive services to our clients.
Fig.2 Schematic for the hydrolysis and preparation of each monosaccharide class (neutrals, amine sugars, sialic acids) prior to LC-MS analysis. (Lowenthal, 2015)
CE is suitable for both derivatized and underivatized carbohydrates and it is established as a powerful and versatile separation tool because of the brief analysis time, the ability to handle very complex glycan mixtures with high sensitivity, the minute quantities of samples and reagent required, the capacity to resolve isomeric glycans, and the possibility to acquire high resolution obtained under physiological conditions. Creative Biolabs owns a team of technical experts to deal with any problem you may meet in CE.
Creative Biolabs is a leading company that has developed a panel of technologies to offer the best monosaccharides analysis service. Our platform provides various options, from which you can always find the best match for your particular research. Please feel free to contact us for more information.