Plasma Glycoprofiling Service
Advancing Disease Detection: Professional Plasma Glycoprofiling Service at Creative Biolabs
Plasma proteins are mostly N- or O-glycosylated. Glycosylation changes are closely related to different diseases, and multiple studies have shown that changes in glycosylation are observed in the glycocalyx of cancer cells. This discovery has prompted scientists to focus on studying new biomarkers according to glycosylation changes in biological samples for early detection of cancer. Advanced glycan analysis technologies are applied to the detection of various biological samples. In recent years, with the rapid development of analytical technology and instruments, especially the progress in mass spectrometry (MS), glycan analysis of various sources has become reliable and efficient. Creative Biolabs has first-class glycan analysis technology and provides diversified biological sample analysis services to clients around the world. For example: Serum, plasma, Urine, and other biological samples.
We provide professional plasma glycoprofiling service, which usually requires a series of steps to be performed on the tested plasma samples.
First, solid particles and cell residues in plasma samples are removed by centrifugation.
The plasma sample is then mixed with ammonium bicarbonate and sugar alcohol aqueous solution, glycosidase is added to promote the release of glycans, and the mixture is incubated at 37°C. Proteins are then removed from the glycans using ethanol precipitation, ultrafiltration, or other protein removal techniques to allow for better analysis of the glycans.
We centrifuge the mixture, take the supernatant and dry it under vacuum, then reconstitute the dried sample with pure water, and use affinity chromatography, gel filtration, ion exchange chromatography, and other methods to analyze the glycans in plasma. Purification and enrichment are performed to improve detection sensitivity and specificity.
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Hydrolysis or enzymatic hydrolysis
Based on actual detection needs, we will perform enzymatic or hydrolysis on the enriched glycan sample to obtain smaller monosaccharide units or specific glycan fragments.
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Glycan analysis
The glycan analysis in plasma is performed using a variety of technologies, including the following:
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Chromatography: such as gel permeation chromatography (GPC) and high-performance liquid chromatography (HPLC), which can perform qualitative and quantitative analysis of glycans.
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Mass spectrometry technologies: such as time-of-flight mass spectrometry (TOF-MS) can be used for structural identification and analysis of glycans.
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Nuclear magnetic resonance (NMR): It provides structural information about glycans, including bonding methods, conformations, and intermolecular interactions.
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Glycomic technology: It is used to study and analyze carbohydrate compounds in biological samples and can help identify and characterize glycans in plasma.
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Fluorescent labeling technology: Fluorescent labeling agents are used to label glycans, and then the glycans are detected and analyzed through fluorescence spectroscopy.
These techniques are applied individually or in combination to provide an in-depth analysis of the composition, structure, and functions of glycans in plasma, providing important information for research.
Publication
Technology: Capillary zone electrophoresis combined with electrospray ionization mass spectrometry (CZE-ESI-MS)
Journal: Analytical Chemistry
IF: 8.008
Published: 2021
Results: The authors used malto-oligosaccharide standards to develop a highly sensitive CZE-ESI-MS detection method. Glycans released in serum (IgG), extracellular vesicles (EVs), and plasma were analyzed qualitatively and quantitatively. The analysis resulted in a unique N-glycan profile signature for each sample type, providing important structural and quantitative information on the glycan profile in relevant diseases.
Fig.1 Glycosylation analysis results of human and canine serum samples.1
Advantages
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Comprehensive service: We provide a full range of plasma glycoprofiling services from sample collection to data interpretation to meet the various needs of clients.
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Advanced technology: We provide advanced bio-spectroscopy technologies, including mass spectrometry and nuclear magnetic resonance, for precise data collection and analysis to support plasma glycoprofiling.
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Personalized solutions: We custom plasma glycoprofiling solutions according to client needs and flexibly respond to different research purposes and needs.
Application
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Research on disease mechanisms: Plasma glycoprofiling provides an in-depth understanding of the pathogenesis and physiological processes of the disease, and provides important clues for research in related fields.
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Drug development: Plasma glycoprofiling is used to evaluate the safety and effectiveness of drug candidates and provides support for drug clinical trials.
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Cancer monitoring: Plasma glycoprofiling is also used for early diagnosis of cancer, monitoring of treatment effects, and assessment of prognosis. By detecting metabolites in plasma, the metabolic characteristics of cancer cells are revealed, providing an important reference for cancer diagnosis and treatment.
Creative Biolabs continues to track the development of Glycoprotein Analysis technology, continuously improves analysis methods and workflows, and always maintains a leading position in technology. Creative Biolabs is committed to offering clients comprehensive and efficient plasma glycoprofiling services. Please feel free to contact us if you need high-quality glycoprofiling, we are your loyal and reliable partner.
Reference
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Marie, Anne-Lise, et al. "High-sensitivity glycan profiling of blood-derived immunoglobulin G, plasma, and extracellular vesicle isolates with capillary zone electrophoresis-mass spectrometry." Analytical chemistry 93.4 (2021): 1991-2002.
For Research Use Only.
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